Our invited talk at the WORKSHOP on AUSTRALIAN BIOMEASUREMENT:
Standards Australia, Sydney, New South Wales, October 26, 2007.
Hosted by the
National Measurement Institute, Australia.
“ROTOR-GENE 3000 and 6000 - BEHIND THE SCENES"
Summary—In part one, we discuss the temperature calibration and adjustment
methods used by
Corbett Research in its Rotor-Gene* family of thermal cyclers.
Because these methods use remote sensing of fluorescence, they can be
performed in the field or in the factory using only standard reference materials.
Topics include rarely discussed aspects of the OTV** liquid crystal technique,
which is patent pending (US20060077311, EP1514084). In part two, we present
a new quantitative model of DNA amplification during the Polymerase Chain
Reaction process. The new model is derived from reaction kinetics. We discuss
some implications for DNA quantitation and thermal cycler design.
* Rotor-Gene is a registered trademark of Corbett Research
** OTV is a trademark of Corbett Research